Institut für Virologie und Immunologie (Institute of Virology and Immunology)

2. Emplacement de l’installation (indiquer l’adresse et les coordonnées géographiques)
Institut für Virologie und Immunologie, Bundesamt für Lebensmittelsicherheit und Veterinärwesen, Eidgenössisches Departement des Innern, Sensemattstrasse 293, CH-3147 Mittelhäusern, Switzerland

N 46° 52' 50.20", E 7° 21' 46.81"

3. Superficie des secteurs de laboratoire, par niveau de confinement
ABL 3 Ag , 10.446.00 m2
Of note, BSL3Ag facilities have special features not comparable to standard BSL3 or BSL4 facilities. The shell is considered BSL4, whereas inside the containment area BSL1, BSL2 and BSL3 space is common standard. Personnel enters through a shower barrier and is provided with suitable laboratory clothing for BSL1, 2 and 3 inside the containment area. Personnel has to shower out when leaving the containment area and has to keep a 72h quarantine (no contact to cloven hoofed animals). The IVI fulfills the requirements of the EU Minimum Biorisk Management Standards for Laboratories Working with Foot-And-Mouth Disease Virus. Due to these special features of BSL3Ag facilities, the BSL3Ag area is not limited to laboratory units only, but also includes engineering floors such as effluent treatment plant or ventilation units and animal units, which are all located within the containment area. Therefore, all maintenance work has to be done during operation – the facility has never been shut down so far. This also means that a direct comparison with BSL4 facilities is not practicable. Licenses are as follows: “Quality controls of immuno-biological products for use in applications of veterinary medicine”; “Establishment of a cell-based rapid test to determine protection provided by vaccination against foot-and-mouth disease virus”; “Validation of decontamination by H2O2”; “Diagnostics of viral pathogens causing highly contagious animal diseases“.
BL 3 , 44.00 m2
BL 2 , 210.00 m2
Superficie totale des laboratoires (m(2))

4. Organigramme de chaque installation

(viii) Décrire brièvement la politique adoptée en matière de publication dans l’installation.

Publication in open literature.

(ix) Fournir une liste des documents et rapports accessibles au public qui portent sur les travaux publiés au cours des douze mois écoulés (indiquer les auteurs, les titres et les références complètes).

Alves, M.P., Vielle, N.J., Thiel, V., and Pfaender, S. (2018) Research models and tools for the identification of antivirals and therapeutics against Zika virus infection. Viruses-Basel 10:11, 593.

Amini, P., Stojkov, D., Felser, A., Jackson, C.B., Courage, C., Schaller, A., Gelman, L., Soriano, M.E., Nuoffer, J.M., Scorrano, L., Benarafa, C., Yousefi, S., and Simon, H.U. (2018) Neutrophil extracellular trap formation requires OPA1-dependent glycolytic ATP production. Nature Communications 9:1, 2958.

Ayrle, H., Mevissen, M., Kaske, M., Vögtlin, A., Fricker, R., Hoffmann, B., Büttner, M., Marinovic, Z., and Walkenhorst, M. (2018) Colostral transmission of BTV-8 antibodies from dairy cows six years after vaccination. Vaccine 36:39, 5807-5810.

Braun, R.O.#, Brunner, L., Wyler, K., Auray, G., García-Nicolás, O., Python, S., Zumkehr, B., Gaschen, V., Stoffel, M.H., Collin, N., Barnier-Quer, C., Bruggmann, R., and Summerfield, A. (2018) System immunology-based identification of blood transcriptional modules correlating to antibody responses in sheep. NPJ Vaccines 3, 41.

Braun, U., Janett, F., Züblin, S., von Büren, M., Hilbe, M., Zanoni, R., and Schweizer, M. (2018) Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses. BMC Veterinary Research 14:1, 159.

Bürgi, N., Josi, C., Bürki, S., Schweizer, M. and Pilo, P. (2018) Mycoplasma bovis co‑infection with bovine viral diarrhea virus in bovine macrophages. Veterinary Research 49, 2.

Devinsky, O., Boesch, J.M., Cerda-Gonzalez, S., Coffey, B., Davis, K., Friedman, D., Hainline, B., Houpt, K., Lieberman, D., Perry, P., Prüss, H., Samuels, M.A., Small, G.W., Volk, H., Summerfield, A., Vite, C., Wisniewski, T., and Natterson-Horowitz, B. (2018) A cross-species approach to disorders affecting brain and behaviour. Nature Reviews Neurology 14:11, 677-686.

Englezou, P.C., Sapet, C., Démoulins, T., Milona, P., Ebensen, T., Schulze, K., Guzman, C.A., Poulhes, F., Zelphati, O., Ruggli, N., and McCullough, K.C. (2018) Self-amplifying replicon RNA delivery to dendritic cells by cationic lipids. Molecular Therapy. Nucleic acids 12, 118-134.

Fernández, M., Braun, U., Frei, S., Schweizer, M., and Hilbe, M. (2018) Border disease virus infection of bovine placentas. Veterinary Pathology 55:3, 425-433.

García-Nicolás, O., Braun, R.O., Milona, P., Lewandowska, M., Dijkman, R., Alves, M.P., and Summerfield, A. (2018) Targeting of the nasal mucosa by Japanese encephalitis virus for non-vector-borne transmission. Journal of Virology 92:24, e01091-18.

García-Nicolás, O., V'kovski, P.#, Vielle, N.J., Ebert, N., Züst, R., Portmann, J., Stalder, H., Gaschen, V., Vieyres, G., Stoffel, M., Schweizer, M., Summerfield, A., Engler, O., Pietschmann, T., Todt, D., Alves, M.P., Thiel, V., and Pfaender, S. (2018) The small compound inhibitor K22 displays broad antiviral activity against different members of the family Flaviviridae and offers potential as pan-viral inhibitor. Antimicrobial Agents and Chemotherapy 62:11, e01206-18.

Hufsky, F., Ibrahim, B., Beer, M., Deng, L., Le Mercier, P., McMahon, D.P., Palmarini, M., Thiel, V., and Marz, M. (2018) Virologists - heroes need weapons. PLoS Pathogens 14:2, e1006771.

Hüsser, L., Pauli, U., and Summermatter, K. (2018) Autoclaving contaminated liquids: no problem? Applied Biosafety 23:2, 96-99.

Ibrahim, B., McMahon, D.P., Hufsky, F., Beer, M., Deng, L., Mercier, P.L., Palmarini, M., Thiel, V., and Marz, M. (2018) A new era of virus bioinformatics. Virus Research 251, 86-90.

Kojima, K., Booth, C.M., Summermatter, K., Bennett, A., Heisz, M., Blacksell, S.D., and McKinney, M. (2018) Risk-based reboot for global lab biosafety. Science 360:6386, 260-262.

Krischuns, T., Günl, F., Henschel, L., Binder, M., Willemsen, J., Schloer, S., Rescher, U., Gerlt, V., Zimmer, G., Nordhoff, C., Ludwig, S., and Brunotte, L. (2018) Phosphorylation of TRIM28 enhances the expression of IFN-β and proinflammatory cytokines during HPAIV infection of human lung epithelial cells. Frontiers in Immunology 9, 2229.

Kümin, D., Gsell Albert, M., and Summermatter, K. (2018) Comparison and validation of three fumigation methods to inactivate foot-and-mouth disease virus. Applied Biosafety 23:2, 70-76.

Locher, S., Schweneker, M., Hausmann, J., and Zimmer, G. (2018) Immunogenicity of propagation-restricted vesicular stomatitis virus encoding Ebola virus glycoprotein in guinea pigs. Journal of General Virology 99:7, 866-879.

Lussi, C., Sauter, K.-S., and Schweizer, M. (2018) Homodimerisation-independent cleavage of dsRNA by a pestiviral nicking endoribonuclease. Scientific Reports 8:1, 8226.

Mazel-Sanchez, B., Boal Carvalho, I., Silva, F., Dijkman, R., and Schmolke, M. (2018) H5N1 influenza A virus PB1-F2 relieves HAX-1-mediated restriction of avian virus polymerase PA in human lung cells. Journal of Virology 92:11, e00425-18.

McElhinney, L.M., Marston, D.A., Wise, E.L., Freuling, C.M., Bourhy, H., Zanoni, R., Moldal, T., Kooi, E.A., Neubauer-Juric, A., Nokireki, T., Muller, T., and Fooks, A.R. (2018) Molecular epidemiology and evolution of European Bat Lyssavirus 2. International Journal of Molecular Sciences 19:1, 156.

Milewska, A., Kindler, E., V'kovski, P., Zeglen, S., Ochman, M., Thiel, V., Rajfur, Z., and Pyrc, K. (2018) APOBEC3-mediated restriction of RNA virus replication. Scientific Reports 8:1, 5960.

Moeschler, S., Locher, S., and Zimmer, G. (2018) 1-Benzyl-3-cetyl-2-methylimidazolium iodide (NH125) is a broad-spectrum inhibitor of virus entry with lysosomotropic features. Viruses-Basel 10:6, 306.

Muth, D., Corman, V.M., Roth, H., Binger, T., Dijkman, R., Gottula, L.T., Gloza-Rausch, F., Balboni, A., Battilani, M., Rihtarič, D., Toplak, I., Ameneiros, R.S., Pfeifer, A., Thiel, V., Drexler, J.F., Müller, M.A., and Drosten, C. (2018) Attenuation of replication by a 29 nucleotide deletion in SARS-coronavirus acquired during the early stages of human-to-human transmission. Scientific Reports 8:1, 15177.

Niemeyer, D., Mösbauer, K., Klein, E.M., Sieberg, A., Mettelman, R.C., Mielech, A.M., Dijkman, R., Baker, S.C., Drosten, C., and Müller, M.A. (2018) The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species. PLoS Pathogens 14:9, e1007296.

Pfaender, S., Helfritz, F.A., Siddharta, A., Todt, D., Behrendt, P., Heyden, J., Riebesehl, N., Willmann, W., Steinmann, J., Münch, J., Ciesek, S., and Steinmann, E. (2018) Environmental stability and infectivity of hepatitis C virus (HCV) in different human body fluids. Frontiers in Microbiology 9, 504.

Rapoport, E.M., Khaidukov, S.V., Gaponov, A.M., Pazynina, G.V., Tsygankova, S.V., Ryzhov, I.M., Belyanchikov, I.M., Milona, P., Bovin, N.V., and McCullough, K.C. (2018) Glycan recognition by human blood mononuclear cells with an emphasis on dendritic cells. Glycoconjugate Journal 35, 191-203.

Rappe, J.C.F., de Wilde, A., Di, H., Müller, C., Stalder, H.P., V'kovski, P., Snijder, E., Brinton, M.A., Ziebuhr, J., Ruggli, N., and Thiel, V. (2018) Antiviral activity of K22 against members of the order Nidovirales. Virus Research 246, 28-34.

Renevey, N., and Thür, B. (2018) Outbreak investigation: how to prevent laboratory contamination during high-throughput testing. Revue Scientifique et Technique - Office International des Epizooties 37:3, 843-856.

Sautter, C.A., Auray, G., Python, S., Liniger, M., and Summerfield, A. (2018) Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4. Developmental and Comparative Immunology 84, 181-192.

Stalder, H.P., Bachofen, C., Schweizer, M., Zanoni, R., Sauerländer, D., and Peterhans, E. (2018) Traces of history conserved over 600 years in the geographic distribution of genetic variants of an RNA virus: Bovine viral diarrhea virus in Switzerland. PLoS ONE 13:12, e0207604.

Talker, S.C., Baumann, A., Barut, G.T., Keller, I., Bruggmann, R., and Summerfield, A. (2018) Precise delineation and transcriptional characterization of bovine blood dendritic-cell and monocyte subsets. Frontiers in Immunology 9, 2505.

Tamò, L., Hibaoui, Y., Kallol, S., Alves, M.P., Albrecht, C., Hostettler, K.E., Feki, A., Rougier, J.S., Abriel, H., Knudsen, L., Gazdhar, A., and Geiser, T. (2018) Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells. American Journal of Physiology - Lung Cellular and Molecular Physiology 315:6, L921-L932.

Tavella, A., Bettini, A., Ceol, M., Zambotto, P., Stifter, E., Kusstatscher, N., Lombardi, R., Nardeli, S., Beato, M.S., Capello, K., and Bertoni, G. (2018) Achievements of an eradication program against caprine arthritis encephalitis virus in South Tyrol, Italy. Veterinary Record 182:2, 51.

Thiel, V. (2018) Synthetic viruses - Anything new? PLoS Pathogens 14:10, e1007019.

Thiel, V., and Berkhout, B. (2018) Virus Research starts publishing the new "Virus Research Consortia Series". Virus Research 257, 119.

van der Hoek, L., Verschoor, E., Beer, M., Höper, D., Wernike, K., Van Ranst, M., Matthijnssens, J., Maes, P., Sastre, P., Rueda, P., Drexler, J.F., Barr, J., Edwards, T., Millner, P., Vermeij, P., de Groof, A., Thiel, V., Dijkman, R., Suter-Riniker, F., Leib, S., Koller, R., Ramette, A., Engler, O., and Beuret, C. (2018) Host switching pathogens, infectious outbreaks and zoonosis: A Marie Skłodowska-Curie innovative training network (HONOURs). Virus Research 257, 120-124.

van Dorland, H.A., Zanoni, R., Gerber, V., Jeannerat, E., Wiederkehr, D., and Burger, D. (2018) Antibody response to Influenza booster vaccination in Franches-Montagnes stallions supplemented with Equi-Strath®: a randomized trial. Veterinary Medicine and Science 4:2, 133-139.

Vielle, N.J., Zumkehr, B., García-Nicolás, O., Blank, F., Stojanov, M., Musso, D., Baud, D., Summerfield, A., and Alves, M.P. (2018) Silent infection of human dendritic cells by African and Asian strains of Zika virus. Scientific Reports 8:1, 5440.

Vreman, S., Auray, G., Savelkoul, H.F.J., Rebel, A., Summerfield, A., and Stockhofe-Zurwieden, N. (2018) Neonatal porcine blood derived dendritic cells subsets show activation after TLR2 or TLR9 stimulation. Developmental and Comparative Immunology 84, 361-370.

Walz, L., Kays, S.-K., Zimmer, G., and von Messling, V. (2018) Sialidase-inhibiting antibody titers correlate with protection from heterologous influenza virus strains of the same neuraminidase subtype. Journal of Virology 92:17, e01006-18.

Yángüez, E., Hunziker, A., Dobay, M.P., Yildiz, S., Schading, S., Elshina, E., Karakus, U., Gehrig, P., Grossmann, J., Dijkman, R., Schmolke, M., and Stertz, S. (2018) Phosphoproteomic-based kinase profiling early in influenza virus infection identifies GRK2 as antiviral drug target. Nature Communications 9:1, 3679.

Zahno ML and Bertoni G. (2018) An immunodominant region of the envelope glyco-protein of small ruminant lentiviruses may function as decoy antigen. Viruses 10, 231.

Pièces jointes
5. Décrire succinctement les travaux sur la défense biologique réalisés dans l’installation, y compris le(s) type(s) de micro-organismes(9) et/ou toxines étudiés, et résumer les études en plein air sur les aérosols biologiques.

The Institute of Virology and Immunology (IVI), which is part of the Swiss Federal Food Safety and Veterinary Office, is the ISO 17025 accredited institute for the diagnosis, surveillance and control of highly contagious epizootics. In addition, the IVI pursues research both on these viruses and emerging viral diseases, as well as their potential transmission to man. The IVI is also the competent authority issuing the licenses required for the sale of veterinary immunobiological products. Basic research is carried out in the fields of immunology and virology, and involves influenza virus, foot-and-mouth disease virus, classical swine fever virus and porcine circovirus type 2. The development and diagnostics branches focus on assays and tests for classical and african swine fever, foot-and-mouth disease, avian influenza, bluetongue, and other highly contagious infectious diseases. In this domain, the IVI occupies a leading position internationally.

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(9) Notamment les virus et prions.