Institut für Medizinische Mikrobiologie (Institute of Medical Microbiology)

2. Emplacement de l’installation (indiquer l’adresse et les coordonnées géographiques)
Institut für Medizinische Mikrobiologie, Medizinische Fakultät, Universität Zürich, Gloriastrasse 30/32, CH-8006 Zürich, Switzerland

N 47° 22' 36.20", E 8° 33' 11.18"

3. Superficie des secteurs de laboratoire, par niveau de confinement
BL 3 , 20.00 m2
Superficie totale des laboratoires (m(2))

4. Organigramme de chaque installation

(viii) Décrire brièvement la politique adoptée en matière de publication dans l’installation.

Publication in peer-reviewed open access literature.

(ix) Fournir une liste des documents et rapports accessibles au public qui portent sur les travaux publiés au cours des douze mois écoulés (indiquer les auteurs, les titres et les références complètes).

The ABC exporter IrtAB imports and reduces mycobacterial siderophores.
Arnold FM, Weber MS, Gonda I, Gallenito MJ, Adenau S, Egloff P, Zimmermann I, Hutter CAJ, Hürlimann LM, Peters EE, Piel J, Meloni G, Medalia O, Seeger MA (2020)
Nature 580: 413-417

Stereocontrolled synthesis of (-)-bactobolin A.
Vojackova P, Michalska L, Necas M, Shcherbakov D, Böttger EC, Sponer J, Sponer J, Svenda J (2020)
J Am Chem Soc 142: 7306-7311

Apralogs: apramycin 5-O-glycosides and ethers with improved antibacterial activity and ribosomal selectivity and reduced susceptibility to the aminoacyltranserferase (3)-IV resistance determinant.
Quirke JCK, Rajasekaran P, Sarpe VA, Sonousi A, Osinnii I, Gysin M, Haldimann K, Fang QJ, Shcherbakov D, Hobbie SN, Sha SH, Schacht J, Vasella A, Böttger EC, Crich D (2020)
J Am Chem Soc 142: 530-544

Treatment of nontuberculous mycobacterial pulmonary disease: an official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline.
Daley CL, Iccarino JM, Lange C, Cambau E, Wallace Jr. RJ, Andrejak C, Böttger EC, Brozek J, Griffith DE, Guglielmetti L, Huitt GA, Knight SL, Leitman P, Marras TK, Oliver KN, Santin M, Stout JE, Tortoli E, van Ingen J, Wagner D, Winthrop KL (2020)
Eur Respir J 56: 2000535

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2.
Custódio TF, Das H, Sheward DJ, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer MA, Gruzinov AY, Jeffries CM, Graewert MA, Svergun DI, Dobrev N, Remans K, Seeger MA, McInerney GM, Murrell B, Hällberg BM, Löw C (2020)
Nat Commun 11: 5588

The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes.
Olombrada M, Peña C, Rodríguez-Galán O, Klingauf-Nerurkar P, Portugal-Calisto D, Oborská-Oplová M, Altvater M, Gavilanes JG, Martínez-Del-Pozo Á, de la Cruz J, García-Ortega L, Panse VG (2020)
Nucleic Acids Res 48: 6210-6222

Biotinylation of membrane proteins for binder selections.
Kuhn BT, Zimmermann I, Hürlimann LM, HutterCAJ , Miscenic C, Dawson RJP, Seeger MA , Geertsma ER (2020)
Methods Mol Biol 2127: 151-165

Generation of synthetic nanobodies against delicate proteins.
Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020)
Nat Protoc 15: 1707-1741

Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes.
Galazzo L, Meier G, Timachi MH, Hutter CAJ, Seeger MA, Bordignon E (2020)
Proc Natl Acad Sci USA 117: 2441-2448

Quorum sensing controls persistence, resuscitation, and virulence of Legionella subpopulations in biofilms.
Personnic N , Striednig B, Hilbi H (2020)
ISME J 15: 196-210

Invasive aspergillosis due to Aspergillus section Usti: a multicenter retrospective study.
Glampedakis E, Cassaing S, Fekkar A, Dannaoui E, Bougnoux ME, Bretagne S, Neofytos D, Schreiber PW, Hennequin C, Morio F, Shadrivova O, Bongomin F, Fernández-Ruiz M, Bellanger AP, Arikan-Akdagli S, Erard V, Aigner M, Paolucci M, Khanna N, Charpentier E, Bonnal C, Brun S, Gabriel F, Riat A, Zbinden R, Le Pape P, Klimko N, Lewis RE, Richardson M, İnkaya AC, Coste AT, Bochud PY, Lamoth F. (2020)
Clin Infect Dis: Online ahead of print

Legionella quorum sensing meets cyclic-di-GMP signaling
Hochstrasser R, Hilbi H (2020)
Curr Opin Microbiol 55: 9-16

Efficacy of EBL-1003 (apramycin) against Acinetobacter baumannii lung infections in mice.
Becker K, Aranzana-Climent V, Cao S, Nilsson A, Shariatgorji R, Haldimann K, Platzack B, Hughes D, Andrén P, Böttger EC, Friberg L, Hobbie S (2020
Clin Microbiol Infect: online ahead of print

The GTPase Nog1 co-ordinates assembly, maturation and quality control of distant ribosomal functional centers.
Klingauf-Nerurkar P, Gillet LC, Portugal-Calisto D, Oplova M, Jäger M, Schubert OT, Pisano A, Peña C, Rao S, Altvater M, Chang Y, Aebersold R, Panse VG (2020)
Elife 9: e52474

Divergent evolution of Legionella RCC1 repeat effectors defines the range of ran GTPase cycle targets.
Swart AL, Steiner B, Gomez-Valero L, Schütz S, Hannemann M, Janning P, Irminger M, Rothmeier E, Buchrieser C, Itzen A, Panse VG, Hilbi H (2020)
mBio 11: e00405-20

Phylogenomics of Mycobacterium africanum reveals a new lineage and a complex evolutionary history.
Coscolla M, Brites D, Menardo F, Loiseau C, Borrell S, Otchere ID, Asante-Poku A, Asare P, Sanchez-Buso L, Gehre F, N'Dira Sanoussi C, Antonio M, Dissou A, Ruiz-Rodriguez P, Fyfe J, Becket P, Niemann S, Alabi AS, Grobusch MP, Kobbe R, Parkhill J, Beisel C, Fenner L, Böttger EC, Meehan CJ, Harris SR, De Jong BC, Yeboah-Manu D, Gagneux S (2020)
Microb Genom: in press

Tentative breakpoints and areas of technical uncertainty for early reading automated disc diffusion for Enterobacterales.
Mancini S, Röthlin K, Bodendörfer E, Herren S, Kolesnik-Goldmann N, Courvalin P, Zbinden R, Böttger EC (2020)
J Antimicrob Chemother 75: 1495-1505

Evaluation of standardized automated rapid antimicrobial susceptibility testing (saRAST) of Enterobacterales-containing blood cultures: a proof-of-principle study.
Mancini S, Bodendörfer E, Kolesnik-Goldmann N, Herren S, Röthlin K, Couvalin P, Böttger EC (2020)
J Antimicrob Chemother 75: 3218-3229

Phosphoinositides and the fate of Legionella in phagocytes
Swart AL, Hilbi H (2020)
Front Immunol 11: 25

Rifabutin is inactivated by Mycobacterium abscessus Arr.
Schäfle D, Selchow P, Borer B, Meuli M, Rominski A, Schulthess B, Sander P (2020)
Antimicrob Agents Chemother: Online ahead of print

KatG as counterselection marker for nontuberculous mycobacteria.
Gagliardi A, Selchow P, Luthra S, Schäfle D, Schulthess B, Sander P (2020)
Antimicrob Agents Chemother 64: e02508-19  

Natural polymorphisms in conferring resistance to delamanid in drug-naïve patients.
Reichmuth ML, Hömke R, Zürcher K, Sander P, Avihingsanon A, Collantes J, Loiseau C, Borrell S, Reinhard M, Wilkinson RJ, Yotebieng M, Fenner L, Böttger EC, Gagneux S, Egger M, Keller PM (2020)
Antimicrob Agents Chemother 64: e00513-20

HIV coinfection is associated with low fitness rpoB variants in rifampicin-resistant Mycobacterium tuberculosis.
Loiseau C, Brites D, Reinhard M, Zürcher K, Borrell S, Ballif M, Fenner L, Cox H, Rutaihwa LK, Wilkinson RJ, Yotebieng M, Carter EJ, Abimiku A, Marcy, Gotuzzo OE, Avihingsanon A, Zetola N, Doulla B, Böttger EC, Egger M, Gagneux S (2020)
Antimicrob Agents Chemother 64: e00782-20

Co-occurence of aminoglycoside and β-lactam resistance mechanisms in aminoglycoside- non-susceptible Escherichia coli isolated in the Zurich area, /> Bodendörfer E, Marchesi M, Imkamp F, Courvalin P, Böttger EC, Mancini S (2020)
Int J Antimicrob Agents 56: 106019

Evolution and function of bacterial RCC1 repeat effectors
Swart AL, Gomez-Valero L, Buchrieser C, Hilbi H (2020)
Cell Microbiol 22: e13246

Mycobacterium marinum produces distinct mycobactin and carboxymycobactin siderophores to promote growth in broth and phagocytes.
Knobloch P, Koliwer-Brandl H, Arnold FM, Hanna N, Gonda I, Adenau S, Personnic N, Barisch C, Seeger MA, Soldati T, Hilbi H (2020)
Cell Microbiol: 22: e13163

Transcriptional responses of dictyostelium discoideum exposed to different classes of bacteria.
Lamrabet O, Melotti A, Burdet F, Hanna N, Perrin J, Nitschke J, Pagni M, Hilbi H, Soldati T, Cosson P (2020)
Front Microbiol 11: 410

Identification of Anti- Mycobacterium and Anti- Legionella Compounds with potential distinctive structural scaffolds from an HD-PBL using phenotypic screens in amoebae host models.
Hanna N, Kicka S, Chiriano G, Harrison C, Ouertatani Sakouhi H, Trofimov V, Kranjc A, Nitschke J, Pagni M, Cosson P, Hilbi H, Leonardo Scapozza L, Soldati T (2020)
Front Microbiol 11: 266

CRHP Finder, a webtool for the detection of clarithromycin resistance in Helicobacter pylori from whole‐genome sequencing data.
Yusibova M, Hasman H, Conradsen Clausen PTL., Imkamp F, Wagner K, Andersen, L.P. (2020)
Helicobacter 25: e12752

Semisynthetic analogs of the antibiotic fidaxomicin-design, synthesis, and biological evaluation.
Dorst A, Berg R, Gertzen CGW, Schäfle D, Zerbe K, Gwerder M, Schnell SD, Sander P, Gohlke H, Gademann K., Dorst A, et al. Among authors: sander p (2020)
ACS Med Chem Let 11: 2414-2420

The structure of the Legionella response regulator LqsR reveals amino acids critical for phosphorylation and dimerization.
Hochstrasser R, Hutter CAJ, Arnold FM, Bärlocher K, Seeger MA, Hilbi H (2020)
Mol Microbiol 113: 1070-1084

Diversity of nontuberculous mycobacteria in Heater-Cooler Devices - results from prospective surveillance.
Kaelin MB, Kuster SP, Hasse B, Schulthess B, Imkamp F, Halbe M, Sander P, Sax H, Schreiber PW (2020)
J Hosp Infect 105: 480-485

International Society of Cardiovascular Infectious Diseases Guidelines for the Diagnosis, Treatment and Prevention of Disseminated Mycobacterium chimaera Infection Following Cardiac Surgery with Cardiopulmonary Bypass.
Hasse B, Hannan M, Keller PM, Maurer FP, Sommerstein R, Mertz D, Wagner D, Fernández-Hidalgo N, Nomura J, Manfrin V, Bettex D, Conte AH, Durante-Mangoni E, Hing-Cheung Tang T, Stuart RL, Lundgren J, Gordon S, Jarashow MC, Schreiber PW, Niemann S, Kohl TA, Daley C, Stewardson AJ, Whitener CJ, Perkins K, Plachouras D, Lamagni T, Chand M, Freiberger T, Zweifel S, Sander P, Schulthess B, Scriven J, Sax H, van Ingen J, Mestres CA, Diekema D, Brown-Elliott BA, Wallace RJ Jr, Baddour LM, Miro JM, Hoen B; M. chimaera ISCVID investigators; Infectious Diseases specialists; Hospital epidemiologists; Microbiologists and molecular typing specialists; Cardiac surgeons/ perfusionists/ cardiologists; Ophthalmology; Anaesthesiologists; Public Health (2020)
J Hosp Infect 104: 214-235

An advanced apralog with increased in-vitro and in-vivo activity toward Gram-negative pathogens and reduced ex-vivo cochleotoxicity
Sonousi A, Quirke J, Waduge P, Janusic T, Gysin M, Haldimann K, Hobbie SN, Sha S, Schacht J, Chow Ch, Vasella A, Böttger EC, Xu S, Crich D (2020)
(highlighted as "VIP Very Important Paper" by the editor)
ChemMedChem: Online ahead of print

Structures of ABC transporters: handle with care
Lewinson O, Orelle C, Seeger MA (2020)
FEBS Lett 594: 3799-3814

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy
Bordignon E, Seeger MA, Galazzo L, Meier G (2020)
FEBS Lett: Online ahead of print

Photodynamic therapy improves skin antisepsis as a prevention strategy in arthroplasty procedures: A pilot study
Waldmann I., Schmid T,Prinz J, Mühleisen B, Zbinden R, Imhof L, Achermann Y (2020)
Photodiagnosis Photodyn Ther 31: 101941

Alpha-defensin lateral flow test does not appear to be useful in predicting shoulder periprosthetic joint infections
Weigelt L, Plate A, Stadler L, Sutter R, Frustaci D, Zbinden R, Zingg PO, Gerber C, Achermann Y (2020)
Int Orthop 44: 1023-1029

Synthesis, antibacterial action and ribosome inhibition of deoxyspectinomycins
Dharuman S, Wilt LA, Liu J, Reeve SM, Thompson CW, Elmore JM, Dimitri Shcherbakov, Lee RB, Böttger EC, Lee RE (2020)
J Antibiot: in press

Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine.
Ghielmetti G, Rosato G, Trovato A, Friedel U, Kirchgaessner C, Perroulaz C, Pendl W, Figi R, Schulthess B, Bloemberg GV, Keller PM, Stephan R, Tortoli E (2020)
Int J Syst Evol Microbiol: Online ahead of print

Synthesis and Biological Evaluation of Iodinated Fidaxomicin Antibiotics
Dorst A, Shchelik IS, Schäfle D, Sander P, Gademann K (2020)
Helv Chim Acta 103: e2000130

High fluence increases the antibacterial efficacy of PACK Cross-Linking
Kling S., Hufschmid FS, Torres-Netto EA.,Randleman JB.,Mark Willcox M., Zbinden R, Hafezi F (2020)
Cornea 39: 1020-1026

SPHN/PHRT: Forming a swiss-wide infrastructure for data-driven sepsis research
Egli A, Battegay M, Büchler AC, Bühlmann P, Calandra T, Eckert P, Furrer H, Greub G, Jakob SM, Kaiser L, Leib SL, Marsch S, Meinshausen N, Pagani JL, Pugin J, Rätsch G, Schrenzel J, Schüpbach R, Siegemund M, Zamboni N, Zbinden R, Zinkernagel A, Borgwardt K (2020)
Stud Health Technol Inform 270: 1163-1167

Pièces jointes
5. Décrire succinctement les travaux sur la défense biologique réalisés dans l’installation, y compris le(s) type(s) de micro-organismes(9) et/ou toxines étudiés, et résumer les études en plein air sur les aérosols biologiques.

The Institute of Medical Microbiology at the University of Zurich is the Regional Competence Center for the primary analysis of bacteriological samples suspicious of a bioterror-related background. This represents an additional and not a continuous task of the diagonstics laboratory proficient in bacteriology, mycology and serology. Research focusses on vaccine and antibiotic development, protein structure analysis, host-pathogen interaction (Legionella, Mycobacteria), antibiotic resistance mechanisms and general aspects of translation.

For further information please visit (website in french):

(9) Notamment les virus et prions.