Téléchargement

    Institut für Mikrobiologie der Bundeswehr (Bundeswehr Institute of Microbiology)

    2. Emplacement de l’installation (indiquer l’adresse et les coordonnées géographiques)
    Neuherbergstraße 11, D-80937 München, Germany

    48°12` N, 11°34 ` E

    3. Superficie des secteurs de laboratoire, par niveau de confinement
    BL 3 , 67.00 m2
    BL 2 , 1.258.00 m2
    Superficie totale des laboratoires (m(2)): 
    1325

    4. Organigramme de chaque installation

    Personnel
    (i) Total des effectifs: 
    71

    (ii) Répartition du personnel

    Militaire: 
    39
    Civil: 
    32

    (iii) Répartition du personnel par catégorie

    Scientifiques: 
    23
    Ingénieurs
    N/A
    Techniciens: 
    42
    Personnel administratif et auxiliaire: 
    6
    (iv) Liste des disciplines scientifiques représentées au sein du personnel scientifique et technique

    Bacteriology, biochemistry, bioinformatics, biotechnology, epidemiology, immunology, medicine, microbiology, molecular biology, veterinary medicine, virology

    (v) Y a-t-il des personnes employées sous contrat dans l’installation? Dans l’affirmative, indiquer leur nombre approximatif.
    20
    Financement
    (vi) Quelles sont la ou les sources de financement de l’activité réalisée dans l’installation? Mentionner si l’activité est entièrement ou partiellement financée par le Ministère de la défense.

    Federal Minsitry of Defence

    (vii) Quels sont les montants des fonds alloués aux secteurs de programme ci-après:

    Recherche: 
    4.2 million EURO, plus 0.2 million EURO caused by the response to coronavirus pandemic.
    Développement: 
    1.9 million EURO
    Essais et évaluation: 
    2 million EURO, plus 0.5 million EURO caused by the response to coronavirus pandemic.
    (viii) Décrire brièvement la politique adoptée en matière de publication dans l’installation.

    Results are published in scientific journals as well as in reports to the Federal Ministry of Defence. Data are also presented in national and international scientific meetings. The Bundeswehr Institute of Microbiology has implemented dual use research of concern (DURC) regulations. Scientists are required to assess the potential of their research before a project is started, during the project period and before results are published.

    (ix) Fournir une liste des documents et rapports accessibles au public qui portent sur les travaux publiés au cours des douze mois écoulés (indiquer les auteurs, les titres et les références complètes).
    • 1.    Sandra Essbauer, Kathrin Baumann, Mathias Schlegel, Michael K Faulde, Res, Jens Lewitzki, Sabine C Sauer, VC, GE Army, Dimitrios Frangoulidis, MD, MC, J M Riehm, DVM (Ret.), Gerhard Dobler, MD, MC, Jens P Teifke, Kdo SanDstBw IV, (Res.), Hermann Meyer, DVM, PhD Vet Corps (Ret.), Rainer G Ulrich, Small Mammals as Reservoir for Zoonotic Agents in Afghanistan, Military Medicine, 2021;, usab008, https://doi.org/10.1093/milmed/usab008
    • 2.    Kalbhenn EM, Bauer T, Stark TD, Knüpfer M, Grass G, Ehling-Schulz M.: Detection and isolation of emetic Bacillus cereus toxin cereulide by reversed phase chromatography. Toxins (Basel);13(2):115. doi: 10.3390/toxins13020115.
    • 3.    Girl, P.; Haut, M.; Riederer,S.; Pfeffer, M.; Dobler, G. Comparison of Three Serological Methods for the Ep-idemiological Investigation of TBE in Dogs. Microorganisms 2021, 9, 399; https://doi.org/10.3390/microorganisms9020399
    • 4.    Müller, K.; Girl, P.; von Buttlar, H.; Dobler, G.; Wölfel, R. Comparison of Two Commercial Surrogate ELISAs to Detect a Neutralising Antibody Response to SARS-CoV-2. Journal of Virological Methods 2021, 292, 114122, doi:10.1016/j.jviromet.2021.114122.
    • 5.    Andrey V. Zhigailov, Alena S. Neupokoyeva, Elina R. Maltseva, Yuliya V. Perfilyeva, Akerke O. Bissenbay, Nurkeldi A. Turebekov, Stefan Frey, Sandra Essbauer, Karlygash S. Abdiyeva, Ye-katerina O. Ostapchuk, Zhanna A. Berdygulova, Rakhan S. Aimbetov, Dinara A. Naizabayeva, Andrey M. Dmitrovskiy, Yuriy A. Skiba, Seidigapbar M. Mamadaliyev: The prevalence of Bor-relia in Ixodes persulcatus in southeastern Kazakhstan, Ticks and Tick-borne Diseases, Vol-ume 12, Issue 4, 2021, doi.org/10.1016/j.ttbdis.2021.101716.
    • 6.    Müller, K.; Girl, P.; Ruhnke, M.; Spranger, M.; Kaier, K.; von Buttlar, H.; Dobler, G.; Borde, J.P. SARS-CoV-2 Seroprevalence among Health Care Workers—A Voluntary Screening Study in a Regional Medical Center in Southern Germany. Int. J. Environ. Res. Public Health 2021, 18, 3910. https://doi.org/10.3390/ijerph18083910
    • 7.    Turebekov, N., Abdiyeva, K., Yegemberdiyeva, R., Kuznetsov, A., Dmitrovskiy, A., Yeraliyeva, L., Shapiyeva, Z., Batyrbayeva, D., Tukhanova, N., Shin, A., Musralina, L., Hoelscher, M., Froeschl, G., Dobler, G., Freimueller, K., Wagner, E., Frey, S., & Essbauer, S. (2021). Oc-currence of Anti-Rickettsia spp. Antibodies in Hospitalized Patients with Undifferentiated Febrile Illness in the Southern Region of Kazakhstan, The American Journal of Tropical Medicine and Hygiene, DOI: 10.4269/ajtmh.20-0388
    • 8.    Rubio-Acero, R., Castelletti, N., Fingerle, V. et al. In Search of the SARS-CoV-2 Protection Correlate: Head-to-Head Comparison of Two Quantitative S1 Assays in Pre-characterized Oligo-/Asymptomatic Patients. Infect Dis Ther (2021). https://doi.org/10.1007/s40121-021-00475-x
    • 9.    Eisermann P, Rubbenstroth D, Cadar D, Thomé-Bolduan C, Eggert P, Schlaphof A, Leypoldt F, Stangel M, Fortwängler T, Hoffmann F, Osterman A, Zange S, Niller HH, Angstwurm K, Pörtner K, Frank C, Wilking H, Beer M, Schmidt-Chanasit J, Tappe D. Active Case Finding of Current Bornavirus Infections in Human En-cephalitis Cases of Unknown Etiology, Germany, 2018-2020. Emerg Infect Dis. 2021 May;27(5):1371-1379. doi: 10.3201/eid2705.204490.
    • 10.    Elsharabassy M, Gummert J, Zange S, Tiefenbacher Cp. A rare case: first description of a patient with meli-oidosis presenting with pericardial effusion in Germany. Clin Res Cardiol. 2021 Apr 23. doi: 10.1007/s00392-021-01860-9.
    • 11.    Kreutzmann T, Schönfeld A, Zange S, Lethaus B. A Case Report of Oculoglandular Tularemia-Chasing Zebras Among Potential Diagnoses. J Oral Maxillofac Surg. 2021 Mar;79(3):629-636. doi: 10.1016/j.joms.2020.08.018.
    • 12.    Müller, K., Girl, P., Giebl, A., von Buttlar, H., Dobler, G., Bugert, J. J., ... & Wölfel, R. (2021). Emerging SARS-CoV-2 variant B. 1.1. 7 reduces neutralisation activity of antibodies against wild-type SARS-CoV-2.Journal of Clinical Virology, 104912. https://doi.org/10.1016/j.jcv.2021.104912
    • 13.    Rank, A.; Tzortzini, A.; Kling, E.; Schmid, C.; Claus, R.; Löll, E.; Burger, R.; Römmele, C.; Dhillon, C.; Müller, K.; et al. One Year after Mild COVID-19: The Majority of Patients Maintain Specific Immunity, but One in Four Still Suffer from Long-Term Symptoms. J. Clin. Med. 2021, 10, 3305. https://doi.org/10.3390/jcm10153305
    • 14.    Lukas Peintner, Edith Wagner, Anna Shin, Nur Tukhanova, Nurkeldi Turebekov, Karlygash Abdiyeva, Olga Spaiser, Yelena Serebrennikova, Erik Tintrup, Andrey Dmitrovskiy, Aliya Zhalmagambetova, Stefan Frey and Sandra Simone Essbauer. Eight Years of Collaboration on Biosafety and Biosecurity Issues Between Kazakhstan and Germany as Part of the German Biosecurity Programme and the G7 Global Partnership Against the Spread of Weapons and Materials of Mass Destruction. Frontiers in Public Health. 2021. DOI: doi.org/10.3389/fpubh.2021.649393
    • 15.    Alexandra Rehn, Peter Braun, Mandy Knüpfer, Roman Wölfel, Markus H Antwerpen, Mathias C Walter, Catching SARS-CoV-2 by Sequence Hybridization: a comparartive Analysis, mSystems 2021 Aug 31; 6(4):e0039221, doi: 10.1128/mSystems.00392-21
    • 16.    Braun P, Rupprich N, Neif D, Grass G. Enzyme-Linked Phage Receptor Binding Protein Assays (ELPRA) Enable Identification of Bacillus anthracis Colonies. Viruses. 2021 Jul 27;13(8):1462. doi: 10.3390/v13081462
    • 17.    Khatamzas E, Muenchhoff M, Rehn A, Graf A, Hellmuth J, Hollaus A, Mohr A-W, Gaitzsch E, Weiglein T, Georgi E, Scherer C, Stecher S-S, Gruetzner S, Blum H, Krebs S, Reischer A, Leutbecher A, Subklewe M, Dick A, Zange S, Girl P, Mueller K, Weigert O, Hopfner K-P, Stemmler H-J, von Bergwelt-Baildon M, Kep-pler O, Wölfel R, Moosmann A, Antwerpen MH. CD8 T cells and antibodies drive SARS-CoV-2 evolution in chronic infection, ResearchSquare 2021 doi: 10.21203/rs.3.rs-846197/v1
    • 18.    Müller, K., Girl, P., Giebl, A. et al. Sensitivity of two SARS-CoV-2 variants with spike protein mutations to neutralising antibodies. Virus Genes (2021). https://doi.org/10.1007/s11262-021-01871-8
    • 19.    Yeh KB, Kairat T, Parek FK, Maltseva E, Yruiy S, Zhanna S, Ablay S, Frey S, Essbauer S, Hewson R, Rich-ards AL, Hay J. Building Scientific Capability and Reducing Biological Threats: The Effect of Three Cooper-ative Bio-Research Programs in Kazakhstan. Frontiers in Public Health. 2021. DOI: 10.3389/fpubh.2021.683192    
    • 20.    Scheiblauer H, Filomena A, Nitsche A , Puyskens A , Corman VM, Drosten C, Zwirglmaier K , Lange C, Emmerich P, Muller M, Knauer O, Nubling CM. Comparative sensitivity evaluation for 122 CE-marked rapid diagnostic tests for SARS-CoV-2 antigen, Germany, September 2020 to April 2021. Euro Surveill 2021: 26 (44), doi.org/10.2807/1560-7917.ES.2021.26.44.2100441
    • 21.    Puyskens A, Krause E, Michel J, Nubling C M, Scheiblauer H, Bourquain D, Grossegesse M, Valusenko R, Corman VM, Drosten C, Zwirglmaier K, Wolfel R, Lange C, Kramer J, Friesen J, Ignatius R, Muller M, Schmidt-Chanasit J, Emmerich P, Schaade L, Nitsche. Establishment of a specimen panel for the decen-tralised technical evaluation of the sensitivity of 31 rapid diagnostic tests for SARS-CoV-2 antigen, Germany, September 2020 to April 2021. Euro Surveill. 2021;26(44), https://doi.org/10.2807/1560-7917.ES.2021.26.44.2100442
    • 22.    Zimmermann F, Urban M, Kruger C, Walter M, Wolfel R, Zwirglmaier K. In vitro evaluation of the effect of mutations in primer binding sites on detection of SARS-CoV-2by RT-qPCR,Journal of Virological Meth-ods(2021),doi:https://doi.org/10.1016/j.jviromet.2021.114352
    • 23.    Tufa TB, Wölfel S, Zubrikova D, Vichova B, Andersson M, Rieß R, Rutaihwa L, Fuchs A, Orth HM, Häussinger D, Feldt T, Popppert S; Dobler G, Bakkes DK, Chitimia-Dobler l. tick species from cattle in the Adama region of Ethiopia and pathogens detected. Exp Appl Acarol 84(2), 459-471. Doi: 10.1007/s10493-021-00623-5.
    • 24.    Schulz A, Barry Y, Stoek F, Pickin MJ, Ba A, Chitimia-Dobler L, Haki ML, Doumbia BA, Eisenbarth A, Di-ambar A, Bah MY, Eiden M, Groschup MH. Detection of Crimean-Congo hemorrhagic fever virus in blood-fed Hyalomma ticks collected from Mauritanian livestock. Parasit Vectors. 2021 Jun 29;14(1):342. doi: 10.1186/s13071-021-04819-x.
    • 25.    Schmitt HJ, Dobler G, Zavadska D, Freimane Z, Fousteris D, Erber W, Jodar L, Palmborg A. TBE Vaccina-tion Breakthrough Cases-Does Age Matter? accines (Basel). 2021 Aug 21;9(8):932. doi: 10.3390/vaccines9080932.
    • 26.    Rubel F, Brugger K, Chitimia-Dobler L, Dautel H, Meyer-Kayser E, Kahl O. Atlas of ticks (Acari: Argasidae, Ixodidae) in Germany. Exp Appl Acarol. 2021 May;84(1):183-214. doi: 10.1007/s10493-021-00619-1.
    • 27.    Rollins RE, Schaper S, Kahlhofer C, Frangoulidis D, Strauß AFT, Cardinale M, Springer A, Strube C, Bakkes DK, Becker NS, Chitimia-Dobler L. Ticks (Acari: Ixodidae) on birds migrating to the island of Ponza, Italy, and the tick-borne pathogens they carry.  Ticks Tick Borne Dis. 2021 Jan;12(1):101590. doi: 10.1016/j.ttbdis.2020.101590
    • 28.    Liebig K, Boelke M, Grund D, Schicht S, Bestehorn-Willmann M, Chitimia-Dobler L, Dobler G, Jung K, Be-cker SC. The Stable Matching Problem in TBEV Enzootic Circulation: How Important Is the Perfect Tick-Virus Match?  Microorganisms. 2021 Jan 19;9(1):196. doi: 10.3390/microorganisms9010196.
    • 29.    Rollins RE, Mouchet A, Margos G, Chitimia-Dobler L, Fingerle V, Becker NS, dingemanse NJ. Repeatable differences in exploratory behaviour predict tick infestation probability in wild great tits. Behavioral Ecolo-gy and Sociobiology. (2021) 75:48. Doi: 10.1007/s00265-021-02972-y.
    • 30.    Lemhöfer G, Chitimia-Dobler L, Dobler G, Bestehorn-Willmann M. Comparison of whole genomes of tick-borne encephalitis virus from mountainous alpine regions and regions with a lower altitude.  Virus Genes. 2021 Apr;57(2):217-221. doi: 10.1007/s11262-020-01821-w.
    • 31.    Laatamna A, Bakkes DK, Chitimia-Dobler L. Morphological anomalies in Rhipicephalus sanguineus s.s. (Acari: Ixodidae) collected from dogs in steppe and high plateaus regions, Algeria.  Exp Appl Acarol. 2021 Apr;83(4):575-582. doi: 10.1007/s10493-021-00599-2
    • 32.    Chitimia-Dobler L, Lindau A, Oehme R, Bestehorn-Willmann M, Antwerpen M, Drehmann M, Hierl T, Ma-ckenstedt U, Dobler G. Tick-Borne Encephalitis Vaccination Protects from Alimentary TBE Infection: Re-sults from an Alimentary Outbreak.  Microorganisms. 2021 Apr 21;9(5):889. doi: 10.3390/microorganisms9050889.
    • 33.    Chitimia-Dobler L, Schaper S, Bröker M, Nava S. Long-Term Itching in a Tourist Following Bite by a Nymph of Dermacentor auratus (Acari: Ixodidae) in Cambodia.  J Med Entomol. 2021 Nov 9;58(6):2495-2498. doi: 10.1093/jme/tjab088.
    • 34.    Bakkes DK, Ropiquet A, Chitimia-Dobler L, Matloa DE, Apanaskevich DA, Horak IG, Mans BJ, Matthee CA. Adaptive radiation and speciation in Rhipicephalus ticks: A medley of novel hosts, nested predator-prey food webs, off-host periods and dispersal along temperature variation gradients.  Mol Phylogenet Evol. 2021 Sep; 162:107178. doi: 10.1016/j.ympev.2021.107178.
    • 35.    Laatamna A, Bakkes DK, Chitimia-Dobler L. Morphological anomalies in Rhipicephalus sanguineus s.s. (Acari: Ixodidae) collected from dogs in steppe and high plateaus regions, Algeria.  Exp Appl Acarol. 2021 Apr;83(4):575-582. doi: 10.1007/s10493-021-00599-2.
    • 36.    Borde JP, Kaier K, Hehn P, Matzarakis A, Frey S, Bestehorn M, Dobler G, Chitimia-Dobler L. The complex interplay of climate, TBEV vector dynamics and TBEV infection rates in ticks-Monitoring a natural TBEV focus in Germany, 2009-2018.  PLoS One. 2021 Jan 7;16(1):e0244668. doi: 10.1371/journal.pone.0244668. eCollection 2021.
    • 37.    Frangoulidis D, Kahlhofer C, Said AS, Osman AY, Chitimia-Dobler L, Shuaib YA. High Prevalence and New Genotype of Coxiella burnetii in Ticks Infesting Camels in Somalia. Pathogens. 2021 Jun 12;10(6):741. doi: 10.3390/pathogens10060741.
    • 38.    Breitkopf VJM, Dobler G, Claus P, Naim HY, Steffen I. IRE1-Mediated Unfolded Protein Response Pro-motes the Replication of Tick-Borne Flaviviruses in a Virus and Cell-Type Dependent Manner.  Viruses. 2021 Oct 27;13(11):2164. doi: 10.3390/v13112164.
    • 39.    Boelke M, Puff C, Becker K, Hellhammer F, Gusmag F, Marks H, Liebig K, Stiasny K, Dobler G, Baumgärtner W, Schulz C, Becker SC. Enteric Ganglioneuritis, a Common Feature in a Subcutaneous TBEV Murine In-fection Model.  Microorganisms. 2021 Apr 18;9(4):875. doi: 10.3390/microorganisms9040875.
    • 40.    Michelitsch A, Fast C, Sick F, Tews BA, Stiasny K, Bestehorn-Willmann M, Dobler G, Beer M, Wernike K. Long-term presence of tick-borne encephalitis virus in experimentally infected bank voles (Myodes glareolus).  Ticks Tick Borne Dis. 2021 Jul;12(4):101693. doi: 10.1016/j.ttbdis.2021.101693.
    Remarques
    N/A
    Pièces jointes
    N/A
    5. Décrire succinctement les travaux sur la défense biologique réalisés dans l’installation, y compris le(s) type(s) de micro-organismes(9) et/ou toxines étudiés, et résumer les études en plein air sur les aérosols biologiques.

    a. Research, development and evaluation of approaches for the rapid detection, identification, differentiation and typing of Orthopox- , Alpha-, Flavi-, Bunya-, Orthomyxo-, Paramyxo-, and Filo- and Coronaviruses as well as Coxiella, Rickettsia, Burkholderia, Yersinia, Brucella, Bacillus, Francisella. Vibrio and Escherichia (EHEC) as well as for the biological toxins Ricin, Abrin, Staphylococcal- enterotoxin-B, Botulinum Neurotoxins and Saxitoxin using state of the art techniques


    b. Establishment of next generation sequencing techniques, sequence data bases and tools for forensic typing


    c. Research, development and evaluation of immunodiagnostics of relevant bacterial and virological agents and biological toxins


    d. Studies of the epidemiology, immunopathogenesis and immune response against Francisella tularensis, Bacillus spp., Burkholderia spp., Brucella spp., Yersinia spp., as well as Corona- and Flaviviruses


    e. Research and evaluation of therapeutic starategies by in vitro studies on the efficacy of antibacterial agents and bacteriophages against bacterial pathogens as well as of antiviral agents against virus infections.


    A list of biological agents and toxins for which diagnostic methods are established and accredited at the Bundeswehr Institute of Microbiology can be obtained using the following link: https://instmikrobiobw.de/fileadmin/user_upload/pdfs/PDF_sample_submissi... No outdoor studies with biological aerosols have been conducted.

    (9) Notamment les virus et prions.

    Index du rapport