Institut für Virologie und Immunologie (Institute of Virology and Immunology)

2. 设施位于何处(包括地址和地理位置)?
Institut für Virologie und Immunologie, Bundesamt für Lebensmittelsicherheit und Veterinärwesen, Eidgenössisches Departement des Innern, Sensemattstrasse 293, CH-3147 Mittelhäusern, Switzerland

N 46° 52' 50.20", E 7° 21' 46.81"

3. 实验室楼面面积,按封闭级别分列
ABL 3 Ag , 10.446.00 平方米
Of note, BSL3Ag facilities have special features not comparable to standard BSL3 or BSL4 facilities. The shell is considered BSL4, whereas inside the containment area BSL1, BSL2 and BSL3 space is common standard. Personnel enters through a shower barrier and is provided with suitable laboratory clothing for BSL1, 2 and 3 inside the containment area. Personnel has to shower out when leaving the containment area and has to keep a 72h quarantine (no contact to cloven hoofed animals). The IVI fulfills the requirements of the EU Minimum Biorisk Management Standards for Laboratories Working with Foot-And-Mouth Disease Virus. Due to these special features of BSL3Ag facilities, the BSL3Ag area is not limited to laboratory units only, but also includes engineering floors such as effluent treatment plant or ventilation units and animal units, which are all located within the containment area. Therefore, all maintenance work has to be done during operation – the facility has never been shut down so far. This also means that a direct comparison with BSL4 facilities is not practicable. Licenses are as follows: “Quality controls of immuno-biological products for use in applications of veterinary medicine”; “Establishment of a cell-based rapid test to determine protection provided by vaccination against foot-and-mouth disease virus”; “Validation of decontamination by H2O2”; “Diagnostics of viral pathogens causing highly contagious animal diseases“; “Peste des petits ruminants virulence“.
BL 3 , 44.00 平方米
BL 2 , 210.00 平方米

4. 每一设施的组织结构:

(八) 简述设施关于发表论文和报告的政策:

Publication in open literature.

(九) 提供一份过去12个月内发表的因工作产生并可公开获得的论文和报告的清单。(请填明作者、标题和全部参考说明。)

Ancar, R., Li, Y., Kindler, E., Cooper, D.A., Ransom, M., Thiel, V., Weiss, S.R., Hesselberth, J.R., and Barton, D.J. (2020) Physiologic RNA targets and refined sequence specificity of coronavirus EndoU. RNA 26:12, 1976-1999. (PubMed) (Artikel)

Auray, G., Talker, S.C., Keller, I., Python, S., Gerber, M., Liniger, M., Ganges, L., Bruggmann, R., Ruggli, N., and Summerfield, A. (2020) High-resolution profiling of innate immune responses by porcine dendritic cell subsets in vitro and in vivo. Frontiers in Immunology 11, 1429. (PubMed) (Artikel)

Barut, G.T.#, Lischer, H.E.L., Bruggmann, R., Summerfield, A., and Talker, S.C. (2020) Transcriptomic profiling of bovine blood dendritic cells and monocytes following toll-like receptor stimulation. European Journal of Immunology 50:11, 1691-1711. (PubMed) (Artikel)

Beyeler, S.#, Steiner, S., Wotzkow, C., Tschanz, S.A., Adhanom Sengal, A., Wick, P., Haenni, B., Alves, M.P., von Garnier, C., and Blank, F. (2020) Multi-walled carbon nanotubes activate and shift polarization of pulmonary macrophages and dendritic cells in an in vivo model of chronic obstructive lung disease. Nanotoxicology 14:1, 77-96. (PubMed) (Artikel)

Blockus, S., Sake, S.M., Wetzke, M., Grethe, C., Graalmann, T., Pils, M., Le Goffic, R., Galloux, M., Prochnow, H., Rox, K., Hüttel, S., Rupcic, Z., Wiegmann, B., Dijkman, R., Rameix-Welti, M.A., Eléouët, J.F., Duprex, W.P., Thiel, V., Hansen, G., Brönstrup, M., Haid, S., and Pietschmann, T. (2020) Labyrinthopeptins as virolytic inhibitors of respiratory syncytial virus cell entry. Antiviral Research 177, 104774. (PubMed) (Artikel)

Cabanelas, E., Panadero, R., Baumman, A., Alves, M.P., Summerfield, A., García-Dios, D., Díaz, P., Remesar, S., Fernández, G., Morrondo, M.P., Díez-Baños, P., and López, C.M. (2020) Cytokine expression in bovine PBMC cultures stimulated with Hypoderma lineatum antigens. Veterinary Parasitology 283, 109165. (PubMed) (Artikel)

Cong, Y., Ulasli, M., Schepers, H., Mauthe, M., V'kovski, P., Kriegenburg, F., Thiel, V., de Haan, C.A.M., and Reggiori, F. (2020) Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle. Journal of Virology 94:4, e01925-19. (PubMed) (Artikel)

Eiden, S., Dijkman, R., Zell, R., Fuchs, J., and Kochs, G. (2020) Using a mouse-adapted A/HK/01/68 influenza virus to analyse the impact of NS1 evolution in codons 196 and 231 on viral replication and virulence. Journal of General Virology 101:6, 587-598. (PubMed) (Artikel)

Eschbaumer, M., Vögtlin, A., Paton, D.J., Barnabei, J.L., Sanchez-Vazquez, M.J., Pituco, E.M., Rivera, A.M., O'Brien, D., Nfon, C., Brocchi, E., Bakkali Kassimi, L., Lefebvre, D.J., Navarro López, R., Maradei, E., Duffy, S.J., Loitsch, A., De Clercq, K., King, D.P., Zientara, S., Griot, C., and Beer, M. (2020) Non-discriminatory exclusion testing as a tool for the early detection of foot-and-mouth disease incursions. Frontiers in Veterinary Science 7, 552670. (PubMed) (Artikel)

Ganges, L., Crooke, H.R., Bohórquez, J.A., Postel, A., Sakoda, Y., Becher, P., and Ruggli, N. (2020) Classical swine fever virus: the past, present and future. Virus Research 289, 198151. (PubMed) (Artikel)

Gasbarri, M., V'kovski, P., Torriani, G., Thiel, V., Stellacci, F., Tapparel, C., and Cagno, V. (2020) SARS-CoV-2 inhibition by sulfonated compounds. Microorganisms 8:12, 1894. (PubMed) (Artikel)

Gultom, M.#, Laloli, L.#, and Dijkman, R. (2020) Well-differentiated primary mammalian airway epithelial cell cultures. Methods in Molecular Biology 2203, 119-134. (PubMed) (Artikel)

Hidber, T., Pauli, U., Steiner, A., and Kuhnert, P. (2020) In vitro and ex vivo testing of alternative disinfectants to currently used more harmful substances in footbaths against Dichelobacter nodosus. PLoS ONE 15:2, e0229066. (PubMed) (Artikel)

Hilty, M., Wüthrich, T.M.#, Godel, A., Adelfio, R., Aebi, S., Burgener, S.S., Illgen-Wilcke, B., and Benarafa, C. (2020) Chronic cigarette smoke exposure and pneumococcal infection induce oropharyngeal microbiota dysbiosis and contribute to long-lasting lung damage in mice. Microbial Genomics 6:12, e000485. (PubMed) (Artikel)

Holwerda, M.#, V'Kovski, P., Wider, M., Thiel, V., and Dijkman, R. (2020) Identification of an antiviral compound from the pandemic response box that efficiently inhibits SARS-CoV-2 infection in vitro. Microorganisms 8:12, 1872. (PubMed) (Artikel)

Hufsky, F., Beerenwinkel, N., Meyer, I.M., Roux, S., Cook, G.M., Kinsella, C.M., Lamkiewicz, K., Marquet, M., Nieuwenhuijse, D.F., Olendraite, I., Paraskevopoulou, S., Young, F., Dijkman, R., Ibrahim, B., Kelly, J., Le Mercier, P., Marz, M., Ramette, A., and Thiel, V. (2020) The International Virus Bioinformatics Meeting 2020. Viruses-Basel 12:12, 1398. (PubMed) (Artikel)

Hüppi, L., Ruggli, N., Python, S., Hoop, R., Albini, S., Grund, C., and Vögtlin, A. (2020) Experimental pigeon paramyxovirus-1 infection in chicken: evaluation of infectivity, clinical and pathological manifestations and diagnostic methods. Journal of General Virology 101:2, 156-167. (PubMed) (Artikel)

Itakura, Y., Matsuno, K., Ito, A., Gerber, M., Liniger, M., Fujimoto, Y., Tamura, T., Kameyama, K.I., Okamatsu, M., Ruggli, N., Kida, H., and Sakoda, Y. (2020) A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis. Virus Research 276, 197809. (PubMed) (Artikel)

Jores, J., Baldwin, C., Blanchard, A., Browning, G.F., Colston, A., Gerdts, V., Goovaerts, D., Heller, M., Juleff, N., Labroussaa, F., Liljander, A., Muuka, G., Nene, V., Nir-Paz, R., Sacchini, F., Summerfield, A., Thiaucourt, F., Unger, H., Vashee, S., Wang, X., and Salt, J. (2020) Contagious bovine and caprine pleuropneumonia: a research community's recommendations for the development of better vaccines. npj Vaccines 5, 66. (PubMed) (Artikel)

Kauer, R.V., Koch, M.C., Schönecker, L., Becker, J., Holwerda, M.#, Glaus, A.N., Hierweger, M.M., Werder, S., Dijkman, R., Meylan, M., and Seuberlich, T. (2020) Fecal shedding of bovine astrovirus CH13/NeuroS1 in veal calves. Journal of Clinical Microbiology 58:5, e01964-19. (PubMed) (Artikel)

Kratzel, A.#, Steiner, S.#, Todt, D., V'kovski, P., Brueggemann, Y., Steinmann, J., Steinmann, E., Thiel, V., and Pfaender, S. (2020) Temperature-dependent surface stability of SARS-CoV-2. Journal of Infection 81:3, 474-476. (PubMed) (Artikel)

Kratzel, A.#, Todt, D., V'kovski, P., Steiner, S.#, Gultom, M.#, Thao, T.T.N.#, Ebert, N., Holwerda, M.#, Steinmann, J., Niemeyer, D., Dijkman, R., Kampf, G., Drosten, C., Steinmann, E., Thiel, V., and Pfaender, S. (2020) Inactivation of severe acute respiratory syndrome coronavirus 2 by WHO-recommended hand rub formulations and alcohols. Emerging Infectious Diseases 26:7, 1592-1595. (PubMed) (Artikel)

Leborgne, N.G.F.#, Taddeo, A., Freigang, S., and Benarafa, C. (2020) Serpinb1a is dispensable for the development and cytokine response of invariant natural killer T cell subsets. Frontiers in Immunology 11, 562587. (PubMed) (Artikel)

Meng, X.J., and Thiel, V. (2020) Emerging and re-emerging porcine viruses [Editorial]. Virus Research 290, 198198. (PubMed) (Artikel)

Meyer, B., Torriani, G., Yerly, S., Mazza, L., Calame, A., Arm-Vernez, I., Zimmer, G., Agoritsas, T., Stirnemann, J., Spechbach, H., Guessous, I., Stringhini, S., Pugin, J., Roux-Lombard, P., Fontao, L., Siegrist, C.A., Eckerle, I., Vuilleumier, N., Kaiser, L., for the Geneva Center for Emerging Viral Diseases (2020) Validation of a commercially available SARS-CoV-2 serological immunoassay. Clinical Microbiology and Infection 26:10, 1386-1394. (PubMed) (Artikel)

Moreno, H., Rastrojo, A., Pryce, R., Fedeli, C., Zimmer, G., Bowden, T.A., Gerold, G., and Kunz, S. (2020) A novel circulating tamiami mammarenavirus shows potential for zoonotic spillover. PLoS Neglected Tropical Diseases 14:12, e0009004. (PubMed) (Artikel)

Nardelli, S., Bettini, A., Capello, K., Bertoni, G., and Tavella, A. (2020) Eradication of caprine arthritis encephalitis virus in the goat population of South Tyrol, Italy: analysis of the tailing phenomenon during the 2016-2017 campaign. Journal of Veterinary Diagnostic Investigation 32:4, 589-593. (PubMed) (Artikel)

Pfaender, S., Mar, K.B., Michailidis, E., Kratzel, A., Boys, I.N., V’kovski, P., Fan, W., Kelly, J.N., Hirt, D., Ebert, N., Stalder, H., Kleine-Weber, H., Hoffmann, M., Heinrich Hoffmann, H., Saeed, M., Dijkman, R., Steinmann, E., Wight-Carter, M., McDougal, M.B., Hanners, N.W., Pöhlmann, S., Gallagher, T., Todt, D., Zimmer, G., Rice, C.M., Schoggins, J.W., and Thiel, V. (2020) LY6E impairs coronavirus fusion and confers immune control of viral disease. Nature Microbiology 5:11, 1330-1339. (PubMed) (Artikel)

Saade, G., Deblanc, C., Bougon, J., Marois-Créhan, C., Fablet, C., Auray, G., Belloc, C., Leblanc-Maridor, M., Gagnon, C.A., Zhu, J., Gottschalk, M., Summerfield, A., Simon, G., Bertho, N., and Meurens, F. (2020) Coinfections and their molecular consequences in the porcine respiratory tract. Veterinary Research 51:1, 80. (PubMed) (Artikel)

Schubert, K., Karousis, E.D., Jomaa, A., Scaiola, A., Echeverria, B., Gurzeler, L.A., Leibundgut, M., Thiel, V., Mühlemann, O., and Ban, N. (2020) SARS-CoV-2 Nsp1 binds the ribosomal mRNA channel to inhibit translation. Nature Structural & Molecular Biology 27, 959-966. (PubMed) (Artikel)

Thao, T.T.N.#, Labroussaa, F., Ebert, N., Jores, J., and Thiel, V. (2020) In-yeast assembly of coronavirus infectious cDNA clones using a synthetic genomics pipeline. Methods in Molecular Biology 2203, 167-184. (PubMed) (Artikel)

Thao, T.T.N.#, Labroussaa, F., Ebert, N., V’kovski, P., Stalder, H.P., Portmann, J., Kelly, J., Steiner, S.#, Holwerda, M.#, Kratzel, A.#, Gultom, M.#, Schmied, K., Laloli, L.#, Hüsser, L., Wider, M., Pfaender, S., Hirt, D., Cippà, V., Crespo-Pomar, S., Schröder, S., Muth, D., Niemeyer, D., Corman, V., Müller, M.A., Drosten, C., Dijkman, R., Jores, J., and Thiel, V. (2020) Rapid reconstruction of SARS-CoV-2 using a synthetic genomics platform. Nature 582:7813, 561-565. (PubMed) (Artikel) (Medienmitteilung)

Thiel, V. (2020) Viral RNA in an m6A disguise. Nature Microbiology 5:4, 531-532. (PubMed) (Artikel)

Thiel, V., and Jores, J. (2020) Ein Klon geht um die Welt. Pipette 2, 16-17. (Artikel)

Trueeb, B.S., Braun, R.O.#, Auray, G., Kuhnert, P., and Summerfield, A. (2020) Differential innate immune responses induced by Mycoplasma hyopneumoniae and Mycoplasma hyorhinis in various types of antigen presenting cells. Veterinary Microbiology 240, 108541. (PubMed) (Artikel)

Usemann, J., Alves, M.P., Ritz, N., Latzin, P., and Müller, L. (2020) Age-dependent response of the human nasal epithelium to rhinovirus infection. European Respiratory Journal 56:5, 2000877. (PubMed) (Artikel)

Vizcaíno Revés, N., Mogel, H.M., Stoffel, M., Summerfield, A., and Forterre, F. (2020) Polarization of macrophages in epidural inflammation induced by canine intervertebral disc herniation. Frontiers in Veterinary Science 7, 32. (PubMed) (Artikel)

V'kovski, P., Steiner, S.#, and Thiel, V. (2020) Proximity labeling for the identification of coronavirus-host protein interactions. Methods in Molecular Biology 2203, 187-204. (PubMed) (Artikel)

Wang, L., Dorn, P., Zeinali, S., Froment, L., Berezowska, S., Kocher, G.J., Alves, M.P., Brügger, M.#, Esteves, B.I.O., Blank, F., Wotzkow, C., Steiner, S., Amacker, M., Peng, R.W., Marti, T.M., Guenat, O.T., Bode, P.K., Moehrlen, U., Schmid, R.A., and Hall, S.R.R. (2020) CD90+CD146+ identifies a pulmonary mesenchymal cell subtype with both immune modulatory and perivascular-like function in postnatal human lung. American Journal of Physiology - Lung Cellular and Molecular Physiology 318:4, L813-L830. (PubMed) (Artikel)

Wang, M., Liniger, M., Muñoz-González, S., Bohórquez, J.A., Hinojosa, Y.#, Gerber, M., López-Soria, S., Rosell, R., Ruggli, N., and Ganges, L. (2020) A poly-uridine insertion in the 3'-untranslated region of classical swine fever virus activates immunity and reduces viral virulence in piglets. Journal of Virology 94:2, e01214-19. (PubMed) (Artikel)

Warryn, L., Dangy, J.P., Gersbach, P., Gehringer, M., Schäfer, A., Ruf, M.T., Ruggli, N., Altmann, K.H., and Pluschke, G. (2020) Development of an ELISA for the quantification of mycolactone, the cytotoxic macrolide toxin of Mycobacterium ulcerans. PLoS Neglected Tropical Diseases 14:6, e0008357. (PubMed) (Artikel)

Zaugg, I., Herrmann, N., and Ottiger, H. (2020) Vaccinovigilance: Gemeldete unerwünschte Wirkungen immunologischer Tierarzneimittel im Jahr 2019. Schweizer Archiv für Tierheilkunde 162:10, 617-624. (PubMed) (Artikel)

Zettl, F., Meister, T.L., Vollmer, T., Fischer, B., Steinmann, J., Krawczyk, A., V'kovski, P., Todt, D., Steinmann, E., Pfaender, S., and Zimmer, G. (2020) Rapid quantification of SARS-CoV-2-neutralizing antibodies using propagation-defective vesicular stomatitis virus pseudotypes. Vaccines (Basel) 8:3, 386. (PubMed) (Artikel)

Zhou, H., Møhlenberg, M., Terczyńska-Dyla, E., Winther, K.G., Hansen, N.H., Vad-Nielsen, J., Laloli, L.#, Dijkman, R., Nielsen, A.L., Gad, H.H., and Hartmann, R. (2020) The IFNL4 gene is a non-canonical interferon gene with a unique but evolutionarily conserved regulation. Journal of Virology 94:5, e01535-19. (PubMed) (Artikel)

5. 简述设施内进行的生物战防御工作,包括研究的微生物1 和/或毒素的类型以及生物气雾剂户外研究。

The Institute of Virology and Immunology (IVI), which is part of the Swiss Federal Food Safety and Veterinary Office, is the ISO 17025 accredited institute for the diagnosis, surveillance and control of highly contagious epizootics. In addition, the IVI pursues research both on these viruses and emerging viral diseases, as well as their potential transmission to man. The IVI is also the competent authority issuing the licenses required for the sale of veterinary immunobiological products. Basic research is carried out in the fields of immunology and virology, and involves influenza virus, foot-and-mouth disease virus, classical swine fever virus and porcine circovirus type 2 as well as coronaviruses. The development and diagnostics branches focus on assays and tests for classical and african swine fever, foot-and-mouth disease, avian influenza, bluetongue, and other highly contagious infectious diseases. In this domain, the IVI occupies a leading position internationally.

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(9) 包括病毒和朊粒。